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Image Search Results
Journal: Translational Cancer Research
Article Title: Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells
doi: 10.21037/tcr-23-2169
Figure Lengend Snippet: Establishment of CHI-resistant cell line. (A) Survival curves of MCF7 and MCF7-CHI-R cells after CHI treatment for 72 hours. (B) Representative bright-field images of MCF7 and MCF7-CHI-R cells. (C) Colony formation of MCF7 and MCF7-CHI-R cells after stained with 0.005% crystal violet. (D) Quantitative determination of colony formation in MCF7 and MCF7-CHI-R cells. (E) The numbers of MCF7 and MCF7-CHI-R cells. (F) Survival curves of MCF7 and MCF7-CHI-R cells after tamoxifen treatment for 72 hours. *, P≤0.05, ***, P≤0.001, vs. MCF7 group; ### , P≤0.001, vs. MCF7-CHI-R group; && , P≤0.01, vs. MCF7 + CHI group. n=3 in each group of each figure. MCF7-CHI-R, CHI-resistant MCF7; MCF7 + CHI, MCF7 cells after treatment with CHI; MCF7-CHI-R + CHI, CHI-resistant MCF7 cells after treatment with CHI. CHI, chidamide.
Article Snippet: In the present study, the
Techniques: Staining
Journal: Translational Cancer Research
Article Title: Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells
doi: 10.21037/tcr-23-2169
Figure Lengend Snippet: Effects of CHI on the cell cycle of MCF7 and MCF7-CHI-R cells. (A) Cell cycle distribution was examined by propidium iodide staining and was analyzed by two-way ANOVA. (B,C) The expression of p21 and p16 after the treatment of CHI was determined by Western blot analysis, and the quantification was analyzed. *, P≤0.05, **, P≤0.01, ***, P≤0.001, vs. MCF7 group; && , P≤0.01, &&& , P≤0.001, vs. MCF7 + CHI group. n=3 in each group of each figure. MCF7-CHI-R, CHI-resistant MCF7; MCF7 + CHI, MCF7 cells after treatment with CHI; MCF7-CHI-R + CHI, CHI-resistant MCF7 cells after treatment with CHI. CHI, chidamide; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ANOVA, analysis of variance.
Article Snippet: In the present study, the
Techniques: Staining, Expressing, Western Blot
Journal: Translational Cancer Research
Article Title: Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells
doi: 10.21037/tcr-23-2169
Figure Lengend Snippet: Effects of CHI on the apoptosis of MCF7 and MCF7-CHI-R cells. (A) Apoptosis was measured by flow cytometry analysis and apoptotic cells rate was analyzed by two-way ANOVA. (B) The expressions of caspase-3 and cleaved caspase-3 after the treatment of CHI were determined by Western blot analysis, and the quantification was analyzed. (C-F) The expression of PARP, cleaved PARP, p53, bax, bcl2, and γH2AX were determined by Western blot analysis, and the quantification was analyzed. *, P≤0.05, **, P≤0.01, ***, P≤0.001, vs. MCF7 group; &&& , P≤0.001, vs. MCF7 + CHI group. n=3 in each group of each figure. MCF7-CHI-R, CHI-resistant MCF7; MCF7 + CHI, MCF7 cells after treatment with CHI; MCF7-CHI-R + CHI, CHI-resistant MCF7 cells after treatment with CHI. PE, phycoerythrin; FITC, fluorescein isothiocyanate; CHI, chidamide; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ANOVA, analysis of variance.
Article Snippet: In the present study, the
Techniques: Flow Cytometry, Western Blot, Expressing
Journal: Translational Cancer Research
Article Title: Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells
doi: 10.21037/tcr-23-2169
Figure Lengend Snippet: Effects of CHI on the ferroptosis of MCF7 and MCF7-CHI-R cells. (A,B) The expression of xCT and GPX4 after the treatment of CHI was determined by Western blot analysis, and the quantification was analyzed. *, P≤0.05, ***, P≤0.001, vs. MCF7 group; & , P≤0.05, && , P≤0.01, vs. MCF7 + CHI group. n=3 in each group of each figure. MCF7-CHI-R, CHI-resistant MCF7; MCF7 + CHI, MCF7 cells after treatment with CHI; MCF7-CHI-R + CHI, CHI-resistant MCF7 cells after treatment with CHI. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CHI, chidamide.
Article Snippet: In the present study, the
Techniques: Expressing, Western Blot
Journal: Translational Cancer Research
Article Title: Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells
doi: 10.21037/tcr-23-2169
Figure Lengend Snippet: Effects of CHI on the oxidative stress of MCF7 and MCF7-CHI-R cells. (A-D) The expression of Keap1, Nrf2, HO-1, and SOD-1 after the treatment of CHI was determined by Western blot analysis, and the quantification was analyzed. (E) Intracellular ROS concentrations were measured in MCF7 and MCF7-CHI-R cells after CHI treatment. *, P≤0.05, **, P≤0.01, ***, P≤0.001, vs. MCF7 group; # , P≤0.05, vs. MCF7-CHI-R group; & , P≤0.05, &&& , P≤0.001, vs. MCF7 + CHI group. n=3 in each group of each figure. MCF7-CHI-R, CHI-resistant MCF7; MCF7 + CHI, MCF7 cells after treatment with CHI; MCF7-CHI-R + CHI, CHI-resistant MCF7 cells after treatment with CHI. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CHI, chidamide; FITC, fluorescein isothiocyanate; ROS, reactive oxygen species.
Article Snippet: In the present study, the
Techniques: Expressing, Western Blot
Journal: Translational Cancer Research
Article Title: Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells
doi: 10.21037/tcr-23-2169
Figure Lengend Snippet: Effects of CHI on the HDAC activity and acetylation of histones H3 and H4 of MCF7 and MCF7-CHI-R cells. (A) The expression of HDAC1 and HDAC10 after the treatment of CHI was determined by Western blot analysis, and the quantification was analyzed. (B-E) The expression of HDAC2, HDAC3, Ac-H3, and Ac-H4 after the treatment of CHI was determined and the quantification was analyzed. *, P≤0.05, **, P≤0.01, ***, P≤0.001, vs. MCF7 group; # , P≤0.05, ## , P≤0.01, vs. MCF7-CHI-R group; && , P≤0.01, &&& , P≤0.001, vs. MCF7 + CHI group. n=3 in each group of each figure. MCF7-CHI-R, CHI-resistant MCF7; MCF7 + CHI, MCF7 cells after treatment with CHI; MCF7-CHI-R + CHI, CHI-resistant MCF7 cells after treatment with CHI. HDAC, histone deacetylase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CHI, chidamide.
Article Snippet: In the present study, the
Techniques: Activity Assay, Expressing, Western Blot, Histone Deacetylase Assay
Journal: Translational Cancer Research
Article Title: Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells
doi: 10.21037/tcr-23-2169
Figure Lengend Snippet: Effects of other chemotherapy drugs on the MCF7 and MCF7-CHI-R cells. (A-H) IC 50 values of ADM, DXT, nab-PTX, PTX, GEM, 5-FU, eribulin, and CTX of MCF7 and MCF7-CHI-R cells. *, P≤0.05, **, P≤0.01, ***, P≤0.001, vs. MCF7 group. n=3 in each group of each figure. MCF7-CHI-R, CHI-resistant MCF7. CHI, chidamide; ADM, doxorubicin; DXT, docetaxel; nab-PTX, albumin-bound paclitaxel; PTX, paclitaxel; GEM, gemcitabine; 5-FU, 5-fluorouracil; CTX, cyclophosphamide; IC 50 , half-maximal inhibitory concentration.
Article Snippet: In the present study, the
Techniques: Concentration Assay